The 64 Main bus route operates between the area of Queen Street East and Wineva Avenue, Main Street Station on the Bloor-Danforth Subway, and the area of Lumsden Avenue and Eastdale Avenue, generally in a north-south direction. Accessible service is provided on the route. Bike racks are available on this route. Main Street Station is an accessible subway station.

One single service is operated: the 64 (Queen-Eastdale) branch operates at all times, seven days a week.

Northbound 64 TO EASTDALE

These buses operate from Wineva Avenue and Queen Street East via west on Queen Street East, north on Hambly Avenue, east on Williamson Road, north on Southwood Drive, north on Main Street, through Main Street Station, continuing north on Main Street, and east on Lumsden Avenue to Eastdale Avenue.

Southbound 64 TO QUEEN

These buses operate from Lumsden Avenue and Eastdale Avenue via south on Eastdale Avenue, west on Secord Avenue, south on Barrington Avenue, west on Doncaster Avenue, south on Main Street, through Main Street Station, continuing south on Main Street, south on Southwood Drive, west on Williamson Road, and south on Wineva Avenue to Queen Street East.

All buses on the route serve local bus stops along the route.