The 41 Keele bus route operates between Keele Station on the Bloor-Danforth Subway, the York University campus, and the area of Steeles Avenue West and Petrolia Road, generally in a north-south direction. Accessible service is provided on the route. Bike racks are available on this route.
Three services are operated. The 41 (Keele Stn-York University and Steeles) is the main branch, and operates during the evening from Monday to Friday, and at all times on Saturdays, Sundays and holidays. The 41B (Keele Stn-Steeles & Petrolia via York University) extended branch operates during the peak periods and midday, from Monday to Friday only. The 41E (Keele Stn- York University Express) express branch operates during the peak periods from Monday to Friday only.

Northbound 41 TO YORK UNIVERSITY AND STEELES
These buses operate from Keele Station via north on Keele Street, east on St. Clair Avenue West, north on Old Weston Road, northwest on Rogers Road, north on Keele Street, north on Trethewey Drive, northeast on Yore Road, north on Keele Street, west on York Boulevard, counter-clockwise through the York University Commons Area, north and west on Ian Macdonald Boulevard, and north on Founders Road to Steeles Avenue West.

Southbound 41 TO KEELE STN
These buses operate from Founders Road and Steeles Avenue West via east on Steeles Avenue West, south on Keele Street, southwest on Yore Road, south on Trethewey Drive, south on Keele Street, southeast on Rogers Road, south on Old Weston Road, west on St. Clair Avenue West, and south on Keele Street to Keele Station.

Northbound 41B TO STEELES & PETROLIA VIA YORK UNIVERSITY
These buses operate from Keele Station via north on Keele Street, east on St. Clair Avenue West, north on Old Weston Road, northwest on Rogers Road, north on Keele Street, north on Trethewey Drive, northeast on Yore Road, north on Keele Street, west on York Boulevard, counter-clockwise through the York University Commons Area, north and west on Ian Macdonald Boulevard, north on Founders Road, east on Steeles Avenue West, and south on Petrolia Road to Canarctic Drive.

Southbound 41B TO KEELE STN
These buses operate from Petrolia Road and Canarctic Drive via west on Canarctic Drive, south on Keele Street, southwest on Yore Road, south on Trethewey Drive, south on Keele Street, southeast on Rogers Road, south on Old Weston Road, west on St. Clair Avenue West, and south on Keele Street to Keele Station.

Northbound 41E TO YORK UNIVERSITY EXPRESS
These buses operate from Keele Station via north on Keele Street, north on Weston Road, east on Rogers Road, north on Keele Street, west on Murray Ross Parkway, north on York University Busway, west on York Boulevard, and counter-clockwise through the York University Commons Area. Northbound 41E buses operate EXPRESS from Keele Station to York University, stopping only at Keele Station, Keele Street & St Clair Avenue, Weston Road & Rogers Road, Keele Street & Rogers Road, Donald Avenue, Eglinton Avenue, Ingram Drive, Lawrence Avenue, Falstaff Avenue, Wilson Avenue, Sheppard Avenue, Finch Avenue (farside), Murray Ross Parkway & York University Busway, and York University Commons Area.

Southbound 41E TO KEELE STN EXPRESS
These buses operate from the York University Commons area via east on York Boulevard, south on York University Busway, east on Murray Ross Parkway, south on Keele Street, west on Rogers Road, south on Weston Road, and south on Keele Street to Keele Station. Southbuond 41E buses operate EXPRESS from York University to Keele Station, stopping only at York University Commons Area,, York University Busway & Murray Ross Parkway, Finch Avenue, Sheppard Avenue, Wilson Avenue, Falstaff Avenue, Lawrence Avenue, Gulliver Road, Eglinton Avenue, Donald Avenue, Keele Street & Rogers Road, Weston Road & Rogers Road, Keele Street & St Clair Avenue (farside), and Keele Station.

All 41 and 41B buses on the route serve local bus stops along the route. The 41E EXPRESS buses stop only at limited stops along the route.