The 38 Highland Creek bus route operates between Scarborough Centre Station on the Scarborough RT, the University of Toronto at Scarborough campus, and the Rouge Hill GO Station, generally in an east-west direction.  Accessible service is provided on the route.  Scarborough Centre Station is an accessible RT station. Bike racks are available on this route.

Two services are operated. The 38 (Scarborough Centre Stn-Rouge Hill GO Stn) branch is the main branch, and operates at all times, seven days a week. The 38A (Scarborough Centre Stn-U of T Scarborough) branch provides supplementary service, and operates at busy times of the week.

Eastbound 38 TO ROUGE HILL GO STN

These buses operate from Scarborough Centre Station via east on Triton Road, south on McCowan Road, east on Ellesmere Road, southeast on Military Trail, east on Old Kingston Road, south over Highland Creek Overpass, east on Lawson Road, south on Port Union Road, and east on Lawrence Avenue East to Rouge Hill GO Station.

Westbound 38 TO SCARBOROUGH CTR

These buses operate from Rouge Hill GO Station via west on Lawrence Avenue East, north on Port Union Road, west on Lawson Road, north over Highland Creek Overpass, west on Highway 2A, northwest on Military Trail, looping south and east through the University of Toronto at Scarborough campus (to Gymnasium stop), continuing northwest on Military Trail, west on Ellesmere Road, north on McCowan Road, and west on Triton Road to Scarborough Centre Station.

Eastbound 38A TO U OF T SCARBOROUGH

These buses operate from Scarborough Centre Station via east on Triton Road, south on McCowan Road, east on Ellesmere Road, southeast on Military Trail to the University of Toronto at Scarborough.

Westbound 38 TO SCARBOROUGH CTR

These buses operate from the University of Toronto at Scarborough campus (to Gymnasium stop) via northwest on Military Trail, west on Ellesmere Road, north on McCowan Road, and west on Triton Road to Scarborough Centre Station.

All buses on the route serve local bus stops along the route.