The 306 Carlton Blue Night streetcar route operates between Main Street Station and Dundas West Station on the Bloor-Danforth Subway, generally in an east-west direction.  Both Main Street Station and Dundas West Station are accessible subway stations.

One single service is operated:  the 306 (Main Street Stn-Dundas West Stn) branch operates during the overnight period, seven days a week.

Westbound 306 DUNDAS WEST STN

These streetcars operate from Main Street Station via south on Main Street, west on Gerrard Street East, south on Coxwell Avenue, continuing west on Gerrard Street East, north on Parliament Street, west on Carlton Street, west on College Street, and northwest and north on Dundas Street West to Bloor Street West (nearside stop).

Eastbound 306 MAIN STREET STN

These streetcars operate from Dundas West Station via east on Edna Avenue, south and southeast on Dundas Street West, east on College Street, east on Carlton Street, south on Parliament Street, east on Gerrard Street East, north on Coxwell Avenue, continuing east on Gerrard Street East, and north on Main Street to Main Street Station.

All streetcars on the route serve local streetcar stops along the route.