The 300 Bloor-Danforth Blue Night bus route operates between the area of Warden Avenue and Danforth Avenue, the area of The West Mall and Burnhamthorpe Road, and Toronto Pearson International Airport, generally in an east-west direction. Bike racks are available on this route.

Two services are operated.  The 300 (Warden-West Mall) is the main branch, and operates during the overnight period, seven days a week.  The 300A (Warden-Pearson Airport) extended branch operates during the overnight period, seven days a week.

Westbound 300 BLUE NIGHT TO WEST MALL

These buses operate from Warden Avenue and Danforth Avenue via west on Danforth Avenue, west on Bloor Street East, west on Bloor Street West, southwest on Dundas Street West, north on Aukland Road, continuing west on Bloor Street West, and north on The West Mall to Burnhamthorpe Road.

Eastbound 300 BLUE NIGHT TO WARDEN

These buses operate from The West Mall and Burnhamthorpe Road via east on Burnhamthorpe Road, south on The East Mall, east on Bloor Street West, south on Aukland Road, east on Dundas Street West, east on Bloor Street West, east on Bloor Street East, east on Danforth Avenue, northeast on Danforth Road, and south on Warden Avenue to Danforth Avenue.

Westbound 300A BLUE NIGHT TO PEARSON AIRPORT

These buses operate from Warden Avenue and Danforth Avenue via west on Danforth Avenue, west on Bloor Street East, west on Bloor Street West, southwest on Dundas Street West, north on Aukland Road, continuing west on Bloor Street West, north on The West Mall, east on Burnhamthorpe Road, north on Highway 427 to Toronto Pearson International Airport, making stops first at Terminal 3 (Arrivals Level), then on the Airport Roadway at Jetliner Road, and then finally at Terminal 1 (Ground Level).

Eastbound 300A BLUE NIGHT TO WARDEN

These buses operate from Pearson Airport Terminal 1 via south on Highway 427, east on Burnhamthorpe Road, south on The East Mall, east on Bloor Street West, south on Aukland Road, east on Dundas Street West, east on Bloor Street West, east on Bloor Street East, east on Danforth Avenue, northeast on Danforth Road, and south on Warden Avenue to Danforth Avenue.

All buses on the route serve local bus stops along the route.