The 26 Dupont bus route operates between St. George Station on the Bloor-Danforth and Yonge-University-Spadina Subways, and Jane Station on the Bloor-Danforth Subway, generally in an east-west direction.  It also serves the Dupont Station on the Yonge-University-Spadina Subway.  Accessible service is provided on the route.  Both St. George and Jane Stations are accessible subway stations. Bike racks are available on this route.

One single service is operated: the 26 (St George Stn-Jane Stn) branch, which operates seven days a week.

Westbound 26 TO JANE STN

These buses operate from St. George Station via north on Bedford Road, west on Dupont Street, west on Annette Street, and south on Jane Street to Jane Station.

Eastbound 26 TO ST GEORGE STN

These buses operate from Jane Station via south on Armadale Avenue, west on Bloor Street West, north on Jane Street, east on Annette Street, east on Dupont Street, south on Bedford Road, and west on Prince Arthur Avenue to St. George Station.

All buses on the route serve local bus stops along the route.