The 101 Downsview Park bus route operates between Downsview Station on the Yonge-University-Spadina Subway and Downsview Park, generally in an east-west direction.  Accessible service is provided on the route.  Downsview Station is an accessible subway station. Bike racks are available on this route.

One single service is operated:  the 101 (Downsview Stn-Parc Downsview Park) branch operates from approximately 6:00 am to 10:00 pm from Monday to Friday, from approximately 9:00 am to 10:00 pm on Saturdays and holidays, and from approximately 9:40 am to 10:00 pm on Sundays, from mid-May to Labour Day; and from approximately 9:00 am to 10:00 pm on Saturdays and holidays, and from approximately 9:40 am to 10:00 pm on Sundays, from Labour Day to mid-May.

Westbound 101 TO DOWNSVIEW PARK

These buses operate from Downsview Station via west on Sheppard Avenue West, south on John Drury Drive, and east on Carl Hall Road, looping clockwise on the Downsview Park roadways around the Toronto Aerospace Museum.

Eastbound 101 TO DOWNSVIEW STN

These buses operate from Downsview Park via west on Carl Hall Road, north on John Drury Drive, and east on Sheppard Avenue West to Downsview Station.

All buses on the route serve local bus stops along the route.